444 In vitro and clinical improvement of sensitive skin functions by ADE-G3 formulations

Sensitive skin is a common condition that can severely impact quality of life. Several mechanisms are thought to be involved, including those affecting the barrier function, hydration and innervation. Our Goal was investigate clinically on in vitro models, benefit formulations dedicated sensitive containing Aquaphilus dolomiae extract-G3 (ADE-G3) function hydration, as well protective responses dry pollution stresses. In sensitized reconstructed human epidermis (RHE) were subjected dehydration stress with or without formulations. Endpoint measurements included transepithelial electric resistance (TEER), protein expression. Clinical water loss (TEWL), pH, lipid index, scores. When applied topically formulations, ADE-G3, increased TEER RHEs. dehydrated RHEs, recovery integrity, evident return ratios filaggrin/pro-filaggrin Caspase-14/pro-Caspase-14 values measured control “natural moisturizing factors”. clinical studies, performed skin, helped improve level maintained over 24 h after application. This an intense sustained moisturization, total lipids esters increase, increase decrease TEWL. Moreover, when exposed by treating RHE benzo[a]pyrene airborne particulate matter (PM10), application prior exposure attenuated induction CYP1A1, CYP1B1 UGT1A7 expression, indicating effect. ADE-G3 but also protected improved integrity cold pollutant induced environments.